Mapeamento de Genes de Resistência do Feijoeiro à Ferrugem, Antracnose e Mancha-Angular Usando Marcadores RAPD*

نویسندگان

  • Fábio G. Faleiro
  • Vilmar A. Ragagnin
  • Ivan Schuster
  • Ronan X. Corrêa
  • Pedro I. Good-God
  • Sérgio H. Brommonshenkel
  • Maurílio A. Moreira
  • Everaldo G. Barros
  • Fabio G. Faleiro
چکیده

Mapping rust, anthracnose and angular leaf spot resistance genes in common bean using RAPD markers Molecular markers were used to study the organization of rust, anthracnose and angular leaf spot resistance genes in common bean (Phaseolus vulgaris) Ouro Negro cultivar. A segregant population of 154 recombinant inbred lines (RIL ́s) from the crossing between Ouro Negro and Rudá cultivars was inoculated under controlled conditions with seven races of Uromyces appendiculatus, three of Colletotrichum lindemuthianum and four of Phaeoisariopsis griseola. DNA samples of each RIL were amplified by polymerase chain reaction using 70 decamer primers. Segregation analysis of rust, anthracnose and angular leaf spot resistance suggested specific resistance inheritance to each physiologic race. Genetic linkage analysis revealed the grouping of different rust and anthracnose resistance genes in the same linkage group. Angular leaf spot resistance genes also were mapped together, but in another linkage group. The utility of RAPD markers linked to common bean resistance genes, previously identified in the literature, was restricted. Only five out of 38 molecular markers tested were validated on the RIL ́s population as linked to rust and anthracnose resistance genes. Three new molecular markers (OBA16669 and OAB16583 to 10.4 cM in coupling and OAD93210 to 13.9 cM in repulsion) were identified that are linked to to the angular leaf spot resistance gene block in Ouro Negro cultivar.

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تاریخ انتشار 2003